IGCSE Biology (0610) — May–June 2025, Paper 13

40 questions · every question answerable online with instant point-by-point AI marking against the official mark scheme.

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Question 1 · 1 mark · Characteristics of living organisms

The Venus flytrap is a plant that feeds on insects. When an insect lands on a specially adapted leaf, the leaf folds very quickly and traps the insect. The leaves produce enzymes…

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Question 2 · 1 mark · Classification and the binomial system

A rat has the scientific name Rattus rattus. What do the two parts of this name refer to? A genus and species B kingdom and genus C kingdom and species D species and group

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Question 3 · 1 mark · Features of organisms — arthropod groups

Which of the animals shown is a crustacean? The diagram shows four animals labelled A to D: A is a winged insect (e.g. a bee/fly with wings and three pairs of legs); B is an…

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Question 4 · 1 mark · Cell structure and organisation

Which features do animal cells share with plant cells? chloroplast cytoplasm nucleus --- --- --- --- A yes yes yes B yes yes no C no yes yes D no no yes

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Question 5 · 1 mark · Size of specimens — magnification

A student makes a drawing of an animal. The length of the animal in the drawing is 30 mm. The actual length of the animal is 200 mm. What is the magnification of the student's…

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Question 6 · 1 mark · Osmosis

Some pieces of potato plant tissue are placed in a very concentrated sugar solution. Other pieces of potato plant tissue are placed in distilled water. What happens to the mass of…

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Question 7 · 1 mark · Active transport

Which statement describes the net movement of particles by active transport in animal cells? A from a higher concentration to a lower concentration across a cell membrane B from a…

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Question 8 · 1 mark · Biological molecules — food tests

Which chemical tests should a student use to test lemon juice for reducing sugars and vitamin C? reducing sugars vitamin C --- --- --- A Benedict's solution test DCPIP solution…

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Question 9 · 1 mark · Enzymes — effect of pH

Which graph shows the effect of pH on enzyme activity? Four graphs of enzyme activity (y-axis) against pH (x-axis) are shown: A is a U-shaped curve (high at low and high pH,…

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Question 10 · 1 mark · Enzymes — effect of temperature

The graph shows the effect of temperature on an enzyme-controlled reaction. At which point on the graph is the enzyme completely denatured? The graph plots rate of reaction…

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Question 11 · 1 mark · Biological molecules — carbohydrates

Which carbohydrate is used to build plant cell walls? A cellulose B glycogen C starch D sucrose

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Question 12 · 1 mark · Leaf structure

In a cross-section of a leaf, what is the correct order of structures moving from the upper surface of the leaf towards the lower surface of the leaf? A cuticle, epidermis,…

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Question 13 · 1 mark · Human nutrition — diet

What is the result of a diet lacking iron? A bleeding gums B poor wound healing C reduced number of red blood cells D weak bones and teeth

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Question 14 · 1 mark · Human nutrition — digestion

The diagram shows part of the human digestive system. Where is lipase made? The diagram shows the upper part of the human digestive system with four labels: A points to the…

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Question 15 · 1 mark · Human nutrition — digestive enzymes

Which row about an enzyme in the alimentary canal is correct? site of action enzyme substrate --- --- --- --- A mouth amylase protein B mouth protease starch C stomach protease…

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Question 16 · 1 mark · Transport in plants — water uptake

What is the pathway taken by water through a plant? A root cortex cells → root hair cells → mesophyll cells → xylem B root cortex cells → root hair cells → xylem → mesophyll cells…

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Question 17 · 1 mark · Transport in animals — circulatory system

Which blood vessel transports blood away from the kidney? A pulmonary artery B pulmonary vein C renal artery D renal vein

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Question 18 · 1 mark · Gas exchange in humans

The diagram shows part of the gas exchange system in humans. The diagram of the thorax shows labels T, U, V and W. W points to the cluster of air sacs (alveoli) at the end of the…

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Question 19 · 1 mark · Respiration — aerobic respiration

Which structure is the site of aerobic respiration in plant cells? A chloroplast B mitochondrion C nucleus D ribosome

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Question 20 · 1 mark · Respiration — anaerobic respiration

Which substance is a product of anaerobic respiration in human muscles? A alcohol B carbon dioxide C glucose D lactic acid

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Question 21 · 1 mark · Excretion in humans — urinary system

The diagram shows a human organ system. The diagram shows the urinary system: two kidneys at the top, connected by tubes to the bladder, which leads to a tube exiting the body at…

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Question 22 · 1 mark · Coordination and response — the eye

The diagram shows a section through the human eye. Which label identifies the iris? The diagram shows a horizontal section through the eye, with four labels A, B, C and D pointing…

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Question 23 · 1 mark · Coordination and response — hormones

Which hormone decreases blood glucose concentration? A adrenaline B glycogen C insulin D oestrogen

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Question 24 · 1 mark · Reproduction — asexual reproduction

What is the result of asexual reproduction? A genetically different offspring from one parent B genetically different offspring from two parents C genetically identical offspring…

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Question 25 · 1 mark · Reproduction in plants — germination

Four test-tubes are set up as shown and placed in the dark. The four test-tubes each contain seeds on cotton wool: A has seeds on moist cotton wool at 0 °C; B has seeds on moist…

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Question 26 · 1 mark · Reproduction — human male reproductive system

Where in the human male reproductive system are sperm produced? A penis B prostate gland C scrotum D testes

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Question 27 · 1 mark · Reproduction — menstrual cycle

A person's menstrual cycle lasts for 28 days. Which statement describes an event in this menstrual cycle? A The ovary releases an egg on day 14. B The ovary releases an egg on day…

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Question 28 · 1 mark · Inheritance — genotype and alleles

An individual has the genotype Bb. What is the correct description of this genotype? A heterozygous, with two different alleles of the same gene B heterozygous, with two different…

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Question 29 · 1 mark · Inheritance — definition

Which statement describes inheritance? A organisms which are not pure-breeding B the difference between homozygous and heterozygous genotypes C the genetic information in dominant…

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Question 30 · 1 mark · Variation and selection — natural selection

What is a feature of natural selection? A genetic variation within populations B plenty of resources C production of genetically identical offspring D selection by humans

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Question 31 · 1 mark · Organisms and their environment — energy flow

What is the principal source of energy input to biological systems? A carbon dioxide in air B glucose C minerals in soil D the Sun

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Question 32 · 1 mark · Organisms and their environment — food webs

The diagram shows a food web. The food web shows: grass is eaten by rabbit, mouse and grasshopper. Rabbit and mouse are eaten by fox; mouse is eaten by owl and hawk; grasshopper…

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Question 33 · 1 mark · Organisms and their environment — carbon cycle

The diagram shows part of the carbon cycle. The diagram shows carbon dioxide gas in the air linked by arrows labelled A, B, C and D to other carbon stores. Arrow A connects fossil…

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Question 34 · 1 mark · Organisms and their environment — population growth

The diagram shows a graph of population growth. The graph plots population size (y-axis) against time (x-axis) as an S-shaped (sigmoid) curve. Region A is the early lag phase…

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Question 35 · 1 mark · Human influences — intensive farming

Two methods of livestock production are described. method 1 Animals are kept in a small area and their movement is restricted. method 2 Animals are allowed to move freely around a…

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Question 36 · 1 mark · Human influences — acid rain / pollution effects

Some species of aquatic insects are able to survive at different ranges of pH. The diagram shows the pH ranges in which four different species are able to survive. The chart shows…

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Question 37 · 1 mark · Biodiversity and conservation

Which practice helps to maintain biodiversity? A improving crop yields by using herbicides B increasing crop yields by using insecticides C the protection of habitats D the use of…

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Question 38 · 1 mark · Human influences — water pollution / eutrophication

The diagram shows four positions on a river where water samples were taken. The diagram shows a river flowing left to right with sampling positions W, X, Y and Z. An untreated…

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Question 39 · 1 mark · Biotechnology and genetic modification — use of bacteria

Which feature makes bacteria useful in genetic modification? A can make complex molecules B have a cell membrane C have a cell wall D reproduce slowly

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Question 40 · 1 mark · Biotechnology and genetic modification — applications

Scientists have developed a genetically modified pineapple plant that produces pink fruit. The pineapple fruit is pink because of increased concentrations of a substance called…

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