IGCSE Biology (0610) — May–June 2025, Paper 21

40 questions · every question answerable online with instant point-by-point AI marking against the official mark scheme.

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Question 1 · 1 mark · Characteristics and classification of living organisms

Which characteristic of living things requires carbon dioxide to diffuse into a leaf? A excretion B movement C nutrition D respiration

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Question 2 · 1 mark · Characteristics and classification of living organisms

Which set of features is characteristic only of birds? A hair and wings B hard-shelled eggs and feathers C scales and soft-shelled eggs D wings and soft-shelled eggs

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Question 3 · 1 mark · Cells - cell structure and organisation

Which structures are found in both plant and animal cells? A cell walls and cell membranes B nuclei and cell walls C cytoplasm and chloroplasts D cell membranes and nuclei

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Question 4 · 1 mark · Movement into and out of cells - active transport

A student made the following statements about the movement of ions by active transport. 1 It is the net movement of particles from a low concentration to a high concentration. 2…

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Question 5 · 1 mark · Biological molecules - DNA structure

A DNA molecule contains pairs of bases. What is a correct combination of a pair of bases? A A and G B C and A C G and T D T and A

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Question 6 · 1 mark · Biological molecules - food tests

What is the test for protein? The table gives, for each row, the name of the test, whether heat is required, and the colour change. name of the test heat colour change --- --- ---…

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Question 7 · 1 mark · Enzymes - effect of temperature

The graph shows how enzyme activity is affected by temperature. The curve of enzyme activity (y-axis) against temperature in degrees C (x-axis, marked 0 to 60) rises from a low…

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Question 8 · 1 mark · Plant nutrition - leaf structure

Which descriptions of adaptations for photosynthesis are correct for spongy mesophyll tissue? The table shows, for each row, whether the tissue has air spaces for efficient gas…

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Question 9 · 1 mark · Human nutrition - diet and deficiency diseases

The table shows the recommended daily allowance (RDA) of some nutrients for young children. The table also shows the masses of these nutrients eaten by a child in one day. mass of…

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Question 10 · 1 mark · Human nutrition - the alimentary canal

Which term is used for the uptake and use of nutrients by cells? A absorption B assimilation C egestion D ingestion

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Question 11 · 1 mark · Human nutrition - chemical digestion

What is the function of trypsin in digestion? A It catalyses the breakdown of maltose in the mouth. B It catalyses the breakdown of maltose in the small intestine. C It catalyses…

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Question 12 · 1 mark · Human nutrition - absorption

The diagram shows the structure of a villus. Four parts are labelled A, B, C and D. A points to the central vessel running up the middle of the villus (the lacteal); B and C point…

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Question 13 · 1 mark · Transport in plants - transpiration

Which graph shows the effect of humidity in the air on the rate of transpiration in a plant? Four graphs of rate of transpiration (y-axis) against percentage humidity (x-axis, 0…

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Question 14 · 1 mark · Transport in plants - translocation

In some countries, spring is the time of year when daffodil plants have green leaves and produce flowers. Which parts of the daffodil plants act as sources and sinks in spring?…

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Question 15 · 1 mark · Transport in animals - circulatory systems

In a fish, what is the sequence of structures that blood passes through after it leaves the heart? A gills - muscles - heart B gills - heart - muscles - heart C muscles - gills -…

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Question 16 · 1 mark · Diseases and immunity - active immunity

The diagram shows what happens in the body during active immunity. A flow chart reads: pathogens enter the body - W are detected - lymphocytes become active, which then branches…

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Question 17 · 1 mark · Diseases and immunity - cholera

Cholera is a disease caused by a bacterium called Vibrio cholerae which produces a toxin in the infected person's gut. What is the effect of this toxin? A The toxin causes loss of…

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Question 18 · 1 mark · Gas exchange in humans - breathing

The graph shows how the volume of air in lungs changes when a person is breathing at rest. The trace is a regular wave of volume of air in lungs (y-axis) against time (x-axis);…

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Question 19 · 1 mark · Respiration - uses of energy

The processes listed occur in living organisms. 1 cell division 2 diffusion 3 muscle contraction 4 osmosis Which processes require energy from respiration? A 1, 2, 3 and 4 B 1 and…

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Question 20 · 1 mark · Excretion in humans - the kidney

What is a role of the glomerulus? A assembling amino acids into proteins B filtration of water, glucose, urea and ions from the blood C reabsorption of glucose, ions and water…

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Question 21 · 1 mark · Coordination and response - synapses

The diagram shows a synapse between neurone 1 (above) and neurone 2 (below). Labels: P points to a vesicle inside the end of neurone 1; Q points to a transmitter substance…

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Question 22 · 1 mark · Coordination and response - the eye

Which row shows the function of rod cells? The table shows, for each row, whether rod cells provide night vision and whether they provide colour vision. night vision colour vision…

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Question 23 · 1 mark · Coordination and response - hormones (adrenaline)

The diagram shows the positions of two endocrine glands in the human body; the enlarged view shows the two glands sitting on top of the kidneys (the adrenal glands). What is a…

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Question 24 · 1 mark · Coordination and response - homeostasis (temperature)

What is a response of the human body to overheating? A vasoconstriction of arterioles B vasoconstriction of veins C vasodilation of arterioles D vasodilation of veins

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Question 25 · 1 mark · Coordination and response - tropic responses

What is an example of phototropism? A the growth of a root in the direction of gravity B the growth of a shoot towards light C the release of energy from glucose using light D the…

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Question 26 · 1 mark · Drugs - antibiotics

Which statement describes the effect of antibiotics? A Antibiotics do not affect bacteria or viruses. B Antibiotics kill bacteria but do not affect viruses. C Antibiotics kill…

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Question 27 · 1 mark · Reproduction - asexual reproduction

The diagram shows one parent Hydra growing and releasing an offspring from the side of its body. Three stages from left to right show a small bud forming on the side of the parent…

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Question 28 · 1 mark · Reproduction - hormones in the menstrual cycle

The diagram shows the changing concentrations of some hormones involved in the menstrual cycle. Hormone concentration (y-axis) is plotted against days 0 to 28 (x-axis). One curve…

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Question 29 · 1 mark · Inheritance - mitosis

The diagram shows the chromosomes in the nucleus of a cell that divides by mitosis. The parent cell nucleus contains six chromosomes (three pairs of differing shapes/lengths).…

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Question 30 · 1 mark · Inheritance - codominance

A farmer bred together male cattle with white hair and female cattle with red hair. All the offspring produced had roan hair (a mixture of red and white). He then repeatedly bred…

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Question 31 · 1 mark · Inheritance - genes and gene expression

Phagocytes and neurones are two types of specialised cell. Which statements are correct? 1 Phagocytes and neurones express the same genes. 2 Both types of cell have the same…

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Question 32 · 1 mark · Organisms and their environment - adaptations / xerophytes

The table shows some features of leaves. Which leaf is adapted to survive in hot, dry habitats? number of stomata thickness of waxy cuticle surface area of leaf --- --- --- --- A…

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Question 33 · 1 mark · Variation and selection - natural and artificial selection

Which row correctly describes a type of selection? type of selection humans involved example --- --- --- --- A artificial no production of farmed animals that produce lots of milk…

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Question 34 · 1 mark · Organisms and their environment - pyramids of energy

Which statement about a pyramid of energy for a food chain is correct? A It shows how energy is lost at each trophic level. B It shows the energy stored at each trophic level. C…

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Question 35 · 1 mark · Organisms and their environment - food webs

The food web shows part of an ocean ecosystem. The producers are kelp, algae and diatom. Primary consumers feeding on producers: sea urchin (eats kelp), mussel and clam (eat…

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Question 36 · 1 mark · Organisms and their environment - the nitrogen cycle

A student investigated the effect of high temperature on the production of nitrate ions in soil. Two samples of soil were taken. One sample was heated to 100 degrees C. All the…

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Question 37 · 1 mark · Organisms and their environment - population growth

Which letter represents the lag phase in the population graph shown? The graph plots number of individuals in the population (y-axis) against time (x-axis) as a sigmoid (S-shaped)…

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Question 38 · 1 mark · Biodiversity and conservation

Which term describes the number of different species living in an area? A biodiversity B conservation C ecosystem D population

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Question 39 · 1 mark · Human influences on ecosystems - eutrophication

Which source of pollution can cause eutrophication? A carbon dioxide B methane C non-biodegradable plastic D sewage

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Question 40 · 1 mark · Biotechnology and genetic modification - producing insulin

Some of the processes involved in the production of insulin by genetic modification are listed. 1 cutting of bacterial plasmid DNA with restriction enzymes 2 expression in…

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