IGCSE Biology (0970) — May–June 2025, Paper 12

40 questions · every question answerable online with instant point-by-point AI marking against the official mark scheme.

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Question 1 · 1 mark · Characteristics and classification of living organisms

When a herbivore sees a carnivore, which characteristic of living things is involved? A excretion B growth C reproduction D sensitivity

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Question 2 · 1 mark · Classification and the binomial system

Which organism has been named using the binomial system? A Brown seaweed B Polar bear C Red fox D Vulpes vulpes

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Question 3 · 1 mark · Features of organisms - arthropods

Which list contains only arthropods? A amphibians, insects, myriapods B arachnids, crustaceans, myriapods C crustaceans, insects, fish D insects, myriapods, reptiles

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Question 4 · 1 mark · Cell structure - plant cell organelles

The diagram shows a plant cell with four labelled structures A, B, C and D. Label A points to the cytoplasm/cell wall region (upper left), B points to a structure on the right…

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Question 5 · 1 mark · Cells - magnification calculations

The table shows the magnifications and image lengths of three specimens when seen with a light microscope. specimen magnification image length / mm --- --- --- X ×50 0.20 Y ×150…

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Question 6 · 1 mark · Movement into and out of cells - osmosis

The diagram represents two liquids separated by a partially permeable membrane. On the left there are many small molecules of water and no large dissolved molecules; on the right…

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Question 7 · 1 mark · Biological molecules - basic units

Small molecules are used to synthesise large food molecules. Which statement is correct? A Amino acids are the basic units of carbohydrates. B Fatty acids are the basic units of…

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Question 8 · 1 mark · Biological molecules - food tests

A student used biuret reagent to test milk for the presence of protein. The milk tested positive for protein. Which colour did the sample change to? A blue B blue-black C brick…

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Question 9 · 1 mark · Photosynthesis - raw materials

Which substances are used for photosynthesis? A carbon dioxide and glucose B carbon dioxide and water C glucose and oxygen D glucose and water

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Question 10 · 1 mark · Plant nutrition - mineral requirements

What happens when a plant lacks magnesium ions? chlorophyll production colour of leaves produced --- --- --- A decreases green B decreases yellow C increases green D increases…

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Question 11 · 1 mark · Leaf structure - tissues

The diagram shows part of a cross-section of a leaf, with three regions bracketed and numbered 1, 2 and 3 from top to bottom. Region 1 is a single layer of cells at the top…

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Question 12 · 1 mark · Human nutrition - balanced diet

A diet contains a lot of plant material, but no meat. Which dietary component is unlikely to be deficient in this diet? A fat B fibre C protein D vitamin D

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Question 13 · 1 mark · Human nutrition - teeth

Which two parts of the mouth are teeth embedded in? A cement and pulp B enamel and dentine C gums and bone D pulp and bone

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Question 14 · 1 mark · Human nutrition - chemical digestion

The diagram shows part of the human digestive system, with four labelled regions. Label D points to the stomach (upper right), label A points to a region near the junction of the…

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Question 15 · 1 mark · Human nutrition - absorption

What is absorbed in the colon and the small intestine? colon small intestine --- --- --- A digested food only digested food and water B digested food only water only C water…

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Question 16 · 1 mark · Transport in plants - transpiration

The diagram shows the movement of water molecules from a plant leaf to its environment. Part of a leaf cross-section is shown with mesophyll cells and a stoma in the lower…

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Question 17 · 1 mark · Transport in animals - circulatory system

The diagram shows a simplified circulatory system in humans as four boxes labelled organ A, organ B, organ C and organ D connected by arrows showing the direction of blood flow.…

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Question 18 · 1 mark · Transport in animals - blood vessels

Which blood vessel transports blood from the lungs to the heart? A aorta B pulmonary artery C pulmonary vein D vena cava

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Question 19 · 1 mark · Diseases and immunity - controlling spread of disease

How can the spread of disease be prevented? A leaving food uncovered B only washing hands after meals C using the same equipment to prepare raw and cooked food D washing hands…

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Question 20 · 1 mark · Gas exchange / respiration - testing for carbon dioxide

Which substance can be used to test for carbon dioxide? A DCPIP B ethanol C iodine solution D limewater

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Question 21 · 1 mark · Respiration - aerobic and anaerobic respiration

Which statement is a correct comparison of aerobic respiration and anaerobic respiration in yeast? A Only aerobic respiration produces carbon dioxide. B Only anaerobic respiration…

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Question 22 · 1 mark · Excretion in humans - urinary system

The diagram shows some of the organs involved in excretion. Two kidneys are shown with tubes leading down to a bladder, which empties through a tube at the bottom. Label A points…

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Question 23 · 1 mark · Coordination and response - reflex action

A person touches a hot object which stimulates structure P in the skin. This causes an electrical impulse to be sent through structure Q to the central nervous system. Which row…

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Question 24 · 1 mark · Coordination and response - hormones

The diagram shows the position of two glands in the body. Gland 1 is shown sitting on top of the left kidney, the right kidney is labelled, the duodenum is labelled, and gland 2…

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Question 25 · 1 mark · Coordination and response - gravitropism

A seedling was placed in a horizontal position. Which diagram shows the expected gravitropic responses in the seedling? Diagram A: both root and shoot remain horizontal. Diagram…

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Question 26 · 1 mark · Reproduction - sperm cell adaptations

The diagram shows a sperm cell with a long tail on the left, a midpiece, and a rounded head on the right. Four parts are labelled: A points to the tail, B points to the…

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Question 27 · 1 mark · Reproduction - sex hormones

Which secondary sexual characteristic is associated with testosterone? A breast development B facial hair growth C start of the menstrual cycle D widening of hips

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Question 28 · 1 mark · Reproduction - menstrual cycle

The diagram shows a human menstrual cycle drawn as a circle marked day 0 (top), day 7 (right), day 14 (bottom) and day 21 (left). Arrow X points to the region just after day 0…

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Question 29 · 1 mark · Inheritance - genes and alleles

What is an alternative form of a gene called? A allele B chromosome C genotype D protein

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Question 30 · 1 mark · Inheritance - monohybrid inheritance

Cystic fibrosis is an inherited condition that occurs when an individual is homozygous for the recessive allele. If both parents are heterozygous for this characteristic, what is…

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Question 31 · 1 mark · Variation and selection - continuous variation

Which characteristic is an example of continuous variation? A ABO blood groups in humans B body mass in cats C seed colour in pea plants D seed shape in pea plants

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Question 32 · 1 mark · Organisms and their environment - adaptation

The photograph shows the butterfly Caligo martia. The butterfly has large patterned wings; the lower (hind) wings each bear a large round dark spot resembling an eye. Which…

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Question 33 · 1 mark · Energy flow - source of energy

What is the principal source of energy input to biological systems? A kinetic B photosynthesis C respiration D the Sun

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Question 34 · 1 mark · Energy flow / food chains - producers

The diagram shows the flow of energy in a food chain as four boxes labelled organism A, organism B, organism C and organism D connected by arrows. Energy flows from organism C up…

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Question 35 · 1 mark · Carbon cycle - respiration

The diagram shows part of the carbon cycle. Boxes show 'carbon dioxide in atmosphere', 'plants', 'animals', and 'waste products and dead organisms'. Numbered arrows: arrow 1 goes…

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Question 36 · 1 mark · Populations - population growth curves

The diagram shows a population curve (a sigmoid growth curve) plotting number of individuals in a population against time. Four points are labelled along the curve: A near the…

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Question 37 · 1 mark · Human impact on ecosystems - biodiversity loss

The graph shows the percentage of species lost, in three different animal groups (mammals; reptiles and amphibians; birds), due to different human activities during the last ten…

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Question 38 · 1 mark · Biotechnology and genetic modification - use of bacteria

Why are bacteria useful in biotechnology and genetic modification? 1 They can make complex molecules. 2 They have a rapid reproduction rate. 3 They have cell walls. A 1, 2 and 3 B…

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Question 39 · 1 mark · Biotechnology - enzymes in washing powders

The graph shows how changing the temperature affects the rate of stain removal from clothes, using a biological washing powder. The curve of rate of stain removal against…

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Question 40 · 1 mark · Biotechnology - biofuel production

The flow chart shows some of the processes involved in making biofuel: grind up plant biomass to extract K → filter to remove waste → add organism L for anaerobic respiration of K…

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