Enzymes — IGCSE Biology (0610) questions by topic

Enzyme questions read a temperature-against-activity graph to find where an enzyme becomes completely denatured, identify the optimum temperature on a similar curve, and choose which of four graphs correctly shows activity rising to a peak before falling sharply once denaturation sets in.

Identify, state, describe, explain and draw lead 42 real questions worth 1 to 23 marks from 2023 to 2025 papers. Because denaturation and optimum temperature are read from very similar-looking curves, submitting a graph-reading answer for instant marking flags whether the peak has been confused with the point of complete denaturation.

May–June 2025, Paper 11

Question 11 · 1 mark

The graph shows the activity of three digestive enzymes at differing pH values. (The graph plots enzyme activity (y-axis) against pH from 1 to 12 (x-axis) for three enzymes.…

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May–June 2025, Paper 13

Question 9 · 1 mark

Which graph shows the effect of pH on enzyme activity? Four graphs of enzyme activity (y-axis) against pH (x-axis) are shown: A is a U-shaped curve (high at low and high pH,…

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Question 10 · 1 mark

The graph shows the effect of temperature on an enzyme-controlled reaction. At which point on the graph is the enzyme completely denatured? The graph plots rate of reaction…

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May–June 2025, Paper 21

Question 7 · 1 mark

The graph shows how enzyme activity is affected by temperature. The curve of enzyme activity (y-axis) against temperature in degrees C (x-axis, marked 0 to 60) rises from a low…

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May–June 2025, Paper 22

Question 8 · 1 mark

What is the correct definition of enzymes? A carbohydrates that act as biological catalysts B carbohydrates that act as substrates C proteins that act as biological catalysts D…

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May–June 2025, Paper 23

Question 5 · 1 mark

The diagram shows a process involving an enzyme in the human stomach. [Diagram: a Pac-Man-shaped enzyme (with a notch) combines with a triangular substrate labelled 1; an…

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May–June 2025, Paper 42

Question 6 · 10 marks

A scientist investigated the effect of changes in pH on the activity of two different protease enzymes. Each enzyme was placed in a hole in the centre of a jelly layer in a Petri…

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May–June 2024, Paper 31

Question 2 · 12 marks

Enzymes are biological catalysts. Describe what is meant by a catalyst.

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May–June 2024, Paper 41

Question 4 · 11 marks

A student investigated the effect of lactase on three different liquids: milk, lactose-free milk, and sucrose solution. The student used an indicator to test for glucose (the…

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October–November 2024, Paper 11

Question 11 · 1 mark

Which type of biological molecule are enzymes made of? A carbohydrate B fat C oil D protein

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Question 12 · 1 mark

The diagrams show molecules involved in the action of a digestive enzyme. What is the substrate? A option A molecule shape B option B molecule shape C option C molecule shape D…

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Question 13 · 1 mark

The graph shows how the activity of an enzyme varies with temperature (rate of enzyme activity vs temperature 0-70 C). What is the optimum temperature for this enzyme, and at…

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October–November 2024, Paper 12

Question 12 · 1 mark

Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are carbohydrates. 3 Enzymes have an active site. A 1 and 2 B 1 and 3 C 2 and 3 D 3 only

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October–November 2024, Paper 13

Question 12 · 1 mark

The diagram represents the shape of an enzyme molecule with an active site. With which substrate would this enzyme most easily form an enzyme-substrate complex? A substrate A B…

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October–November 2024, Paper 21

Question 9 · 1 mark

The graph shows how temperature affects an enzyme-controlled reaction, with points A, B, C and D marked on the curve. Where on the graph do the enzyme and substrate molecules have…

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October–November 2024, Paper 22

Question 9 · 1 mark

The diagram shows the steps in an enzyme-controlled reaction, with a part labelled X (formed when the substrate is bound in the enzyme). What describes the part labelled X? A…

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Question 10 · 1 mark

The graph shows the effect of temperature on the time taken for the complete digestion of starch (time for complete digestion / minutes against temperature 0-60 C); time is…

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October–November 2024, Paper 23

Question 7 · 1 mark

What are the characteristics of an enzyme? (columns: the active site is the same shape as the substrate; it is used up during the reaction; it is made of protein) A no; no; yes B…

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Question 8 · 1 mark

An enzyme in potato cells causes oxygen to be produced from the breakdown of hydrogen peroxide. Cubes of potato tissue were placed in a hydrogen peroxide solution at different…

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October–November 2024, Paper 32

Question 2 · 11 marks

Tick (✓) all the boxes that describe enzymes. The statements are: 'they are permanently changed by the reaction', 'they are involved in all metabolic reactions', 'they are…

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October–November 2024, Paper 53

Question 1 · 23 marks

For this investigation, you need to identify when all of the starch has been broken down in each test-tube. Describe how you can tell if the starch has been broken down.

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October–November 2024, Paper 63

Question 1 · 23 marks

The student needed to identify when all of the starch had been broken down in each test-tube. Describe how the student can tell if the starch has been broken down.

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May–June 2023, Paper 11

Question 10 · 1 mark

What is true of all enzymes? The table has columns: they are sugars; they are most effective at pH 7. Each row A–D shows yes/no for each statement (key: ✓ = yes, blank = no). A…

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Question 11 · 1 mark

The diagram shows enzyme X with four possible molecules A, B, C and D of different shapes near its active site. What is the substrate for enzyme X? (The correct substrate is the…

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May–June 2023, Paper 12

Question 10 · 1 mark

Which processes depend on the action of enzymes? 1 digestion 2 osmosis 3 respiration A 1 and 2 B 1 and 3 C 1 only D 2 and 3

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Question 11 · 1 mark

Which diagram of an enzyme, active site and substrate is correct? Each option A–D shows an enzyme shape with an active site and a substrate; the correct diagram (C) shows the…

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May–June 2023, Paper 13

Question 10 · 1 mark

What is a characteristic of all catalysts? A They are broken down in the reaction. B They are made in the pancreas. C They are not changed by the reaction. D They do not change…

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Question 11 · 1 mark

This sequence of diagrams shows how an enzyme works, with parts labelled P, Q, R and S. The active site is the part of the enzyme into which the substrate fits. Which labelled…

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May–June 2023, Paper 21

Question 9 · 1 mark

What is true of all enzymes? Columns: they are sugars; they are most effective at pH 7 (key: ✓ = yes, blank = no). Which row A–D is correct? A B C D

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Question 10 · 1 mark

Which statement about human enzymes is correct when they are at temperatures above 80 °C? A The enzyme molecules are denatured. B The shape of the active site is stable. C More…

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May–June 2023, Paper 22

Question 10 · 1 mark

The graphs show the effect of temperature on enzyme activity. This enzyme has an optimum temperature of 30 °C. Each option A–D plots enzyme activity against temperature (0–60 °C).…

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May–June 2023, Paper 23

Question 9 · 1 mark

The statements are about enzymes. 1 Enzymes are catalysts. 2 Enzymes are proteins. 3 Enzymes are used up during chemical reactions. Which statements are correct? A 1, 2 and 3 B 1…

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Question 10 · 1 mark

When the temperature increases, the rate of an enzyme-catalysed reaction increases until the optimum temperature is reached. Which statement explains why the rate increases? A The…

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Question 21 · 1 mark

Which graph shows the effect of temperature on enzyme activity? Each option A–D plots enzyme activity against temperature. The correct graph (C) rises to a peak at the optimum…

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May–June 2023, Paper 32

Question 3 · 12 marks

Fig. 3.1 shows the action of an enzyme. On Fig. 3.1 use label lines and labels to identify: an enzyme; a product; a substrate.

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October–November 2023, Paper 11

Question 10 · 1 mark

The graph shows the effect of temperature on the activity of four different enzymes (P, Q, R, S) against temperature (0–110 °C). Which conclusion about the data in the graph is…

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Question 12 · 1 mark

The diagram shows a test-tube at the start and at the end of an enzyme experiment (enzyme + substrate → enzyme + products, after 10 minutes at 35 °C). At the end of the…

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October–November 2023, Paper 12

Question 10 · 1 mark

Which changes could cause an enzyme to denature? 1 change in pH 2 change in temperature 3 change in substrate concentration A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3…

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October–November 2023, Paper 13

Question 10 · 1 mark

The diagrams represent an enzyme and its substrate. The enzyme is shown with four regions labelled A, B, C and D. Which part of the enzyme molecule is the active site for this…

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October–November 2023, Paper 21

Question 10 · 1 mark

Which statement is correct? A The active site of an enzyme has the same shape as the substrate. B The active site is not affected by changes in temperature. C The shape of the…

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Question 12 · 1 mark

The diagram shows a test-tube at the start and at the end of an enzyme experiment (enzyme + substrate → enzyme + products, after 10 minutes at 35 °C). At the end of the…

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October–November 2023, Paper 23

Question 10 · 1 mark

The graph shows the effect of temperature on the rate of a reaction catalysed by an enzyme, with section X on the part of the curve just after the peak (where the rate falls).…

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