Enzymes — IGCSE Biology (0610) questions by topic
Enzyme questions read a temperature-against-activity graph to find where an enzyme becomes completely denatured, identify the optimum temperature on a similar curve, and choose which of four graphs correctly shows activity rising to a peak before falling sharply once denaturation sets in.
Identify, state, describe, explain and draw lead 42 real questions worth 1 to 23 marks from 2023 to 2025 papers. Because denaturation and optimum temperature are read from very similar-looking curves, submitting a graph-reading answer for instant marking flags whether the peak has been confused with the point of complete denaturation.
May–June 2025, Paper 11
The graph shows the activity of three digestive enzymes at differing pH values. (The graph plots enzyme activity (y-axis) against pH from 1 to 12 (x-axis) for three enzymes.…
Answer this question and get it marked →May–June 2025, Paper 13
Which graph shows the effect of pH on enzyme activity? Four graphs of enzyme activity (y-axis) against pH (x-axis) are shown: A is a U-shaped curve (high at low and high pH,…
Answer this question and get it marked →The graph shows the effect of temperature on an enzyme-controlled reaction. At which point on the graph is the enzyme completely denatured? The graph plots rate of reaction…
Answer this question and get it marked →May–June 2025, Paper 21
The graph shows how enzyme activity is affected by temperature. The curve of enzyme activity (y-axis) against temperature in degrees C (x-axis, marked 0 to 60) rises from a low…
Answer this question and get it marked →May–June 2025, Paper 22
What is the correct definition of enzymes? A carbohydrates that act as biological catalysts B carbohydrates that act as substrates C proteins that act as biological catalysts D…
Answer this question and get it marked →May–June 2025, Paper 23
The diagram shows a process involving an enzyme in the human stomach. [Diagram: a Pac-Man-shaped enzyme (with a notch) combines with a triangular substrate labelled 1; an…
Answer this question and get it marked →May–June 2025, Paper 42
A scientist investigated the effect of changes in pH on the activity of two different protease enzymes. Each enzyme was placed in a hole in the centre of a jelly layer in a Petri…
Answer this question and get it marked →May–June 2024, Paper 31
Enzymes are biological catalysts. Describe what is meant by a catalyst.
Answer this question and get it marked →May–June 2024, Paper 41
A student investigated the effect of lactase on three different liquids: milk, lactose-free milk, and sucrose solution. The student used an indicator to test for glucose (the…
Answer this question and get it marked →October–November 2024, Paper 11
Which type of biological molecule are enzymes made of? A carbohydrate B fat C oil D protein
Answer this question and get it marked →The diagrams show molecules involved in the action of a digestive enzyme. What is the substrate? A option A molecule shape B option B molecule shape C option C molecule shape D…
Answer this question and get it marked →The graph shows how the activity of an enzyme varies with temperature (rate of enzyme activity vs temperature 0-70 C). What is the optimum temperature for this enzyme, and at…
Answer this question and get it marked →October–November 2024, Paper 12
Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are carbohydrates. 3 Enzymes have an active site. A 1 and 2 B 1 and 3 C 2 and 3 D 3 only
Answer this question and get it marked →October–November 2024, Paper 13
The diagram represents the shape of an enzyme molecule with an active site. With which substrate would this enzyme most easily form an enzyme-substrate complex? A substrate A B…
Answer this question and get it marked →October–November 2024, Paper 21
The graph shows how temperature affects an enzyme-controlled reaction, with points A, B, C and D marked on the curve. Where on the graph do the enzyme and substrate molecules have…
Answer this question and get it marked →October–November 2024, Paper 22
The diagram shows the steps in an enzyme-controlled reaction, with a part labelled X (formed when the substrate is bound in the enzyme). What describes the part labelled X? A…
Answer this question and get it marked →The graph shows the effect of temperature on the time taken for the complete digestion of starch (time for complete digestion / minutes against temperature 0-60 C); time is…
Answer this question and get it marked →October–November 2024, Paper 23
What are the characteristics of an enzyme? (columns: the active site is the same shape as the substrate; it is used up during the reaction; it is made of protein) A no; no; yes B…
Answer this question and get it marked →An enzyme in potato cells causes oxygen to be produced from the breakdown of hydrogen peroxide. Cubes of potato tissue were placed in a hydrogen peroxide solution at different…
Answer this question and get it marked →October–November 2024, Paper 32
Tick (✓) all the boxes that describe enzymes. The statements are: 'they are permanently changed by the reaction', 'they are involved in all metabolic reactions', 'they are…
Answer this question and get it marked →October–November 2024, Paper 53
For this investigation, you need to identify when all of the starch has been broken down in each test-tube. Describe how you can tell if the starch has been broken down.
Answer this question and get it marked →October–November 2024, Paper 63
The student needed to identify when all of the starch had been broken down in each test-tube. Describe how the student can tell if the starch has been broken down.
Answer this question and get it marked →May–June 2023, Paper 11
What is true of all enzymes? The table has columns: they are sugars; they are most effective at pH 7. Each row A–D shows yes/no for each statement (key: ✓ = yes, blank = no). A…
Answer this question and get it marked →The diagram shows enzyme X with four possible molecules A, B, C and D of different shapes near its active site. What is the substrate for enzyme X? (The correct substrate is the…
Answer this question and get it marked →May–June 2023, Paper 12
Which processes depend on the action of enzymes? 1 digestion 2 osmosis 3 respiration A 1 and 2 B 1 and 3 C 1 only D 2 and 3
Answer this question and get it marked →Which diagram of an enzyme, active site and substrate is correct? Each option A–D shows an enzyme shape with an active site and a substrate; the correct diagram (C) shows the…
Answer this question and get it marked →May–June 2023, Paper 13
What is a characteristic of all catalysts? A They are broken down in the reaction. B They are made in the pancreas. C They are not changed by the reaction. D They do not change…
Answer this question and get it marked →This sequence of diagrams shows how an enzyme works, with parts labelled P, Q, R and S. The active site is the part of the enzyme into which the substrate fits. Which labelled…
Answer this question and get it marked →May–June 2023, Paper 21
What is true of all enzymes? Columns: they are sugars; they are most effective at pH 7 (key: ✓ = yes, blank = no). Which row A–D is correct? A B C D
Answer this question and get it marked →Which statement about human enzymes is correct when they are at temperatures above 80 °C? A The enzyme molecules are denatured. B The shape of the active site is stable. C More…
Answer this question and get it marked →May–June 2023, Paper 22
The graphs show the effect of temperature on enzyme activity. This enzyme has an optimum temperature of 30 °C. Each option A–D plots enzyme activity against temperature (0–60 °C).…
Answer this question and get it marked →May–June 2023, Paper 23
The statements are about enzymes. 1 Enzymes are catalysts. 2 Enzymes are proteins. 3 Enzymes are used up during chemical reactions. Which statements are correct? A 1, 2 and 3 B 1…
Answer this question and get it marked →When the temperature increases, the rate of an enzyme-catalysed reaction increases until the optimum temperature is reached. Which statement explains why the rate increases? A The…
Answer this question and get it marked →Which graph shows the effect of temperature on enzyme activity? Each option A–D plots enzyme activity against temperature. The correct graph (C) rises to a peak at the optimum…
Answer this question and get it marked →May–June 2023, Paper 32
Fig. 3.1 shows the action of an enzyme. On Fig. 3.1 use label lines and labels to identify: an enzyme; a product; a substrate.
Answer this question and get it marked →October–November 2023, Paper 11
The graph shows the effect of temperature on the activity of four different enzymes (P, Q, R, S) against temperature (0–110 °C). Which conclusion about the data in the graph is…
Answer this question and get it marked →The diagram shows a test-tube at the start and at the end of an enzyme experiment (enzyme + substrate → enzyme + products, after 10 minutes at 35 °C). At the end of the…
Answer this question and get it marked →October–November 2023, Paper 12
Which changes could cause an enzyme to denature? 1 change in pH 2 change in temperature 3 change in substrate concentration A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3…
Answer this question and get it marked →October–November 2023, Paper 13
The diagrams represent an enzyme and its substrate. The enzyme is shown with four regions labelled A, B, C and D. Which part of the enzyme molecule is the active site for this…
Answer this question and get it marked →October–November 2023, Paper 21
Which statement is correct? A The active site of an enzyme has the same shape as the substrate. B The active site is not affected by changes in temperature. C The shape of the…
Answer this question and get it marked →The diagram shows a test-tube at the start and at the end of an enzyme experiment (enzyme + substrate → enzyme + products, after 10 minutes at 35 °C). At the end of the…
Answer this question and get it marked →October–November 2023, Paper 23
The graph shows the effect of temperature on the rate of a reaction catalysed by an enzyme, with section X on the part of the curve just after the peak (where the rate falls).…
Answer this question and get it marked →Other IGCSE Biology topics
- Organisms and their environment (126)
- Human nutrition (95)
- Characteristics and classification of living organisms (84)
- Coordination and response (83)
- Reproduction (82)
- Inheritance (74)
- Organisation of the organism (cells) (64)
- Plant nutrition (62)
- Transport in animals (62)
- Transport in plants (59)
- Movement into and out of cells (57)
- Variation and selection (50)