AS & A Level Biology (9700) — May–June 2025, Paper 12

40 questions · every question answerable online with instant point-by-point AI marking against the official mark scheme.

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Question 1 · 1 mark · 1 Cell structure

Which row shows possible uses of an eyepiece graticule? comparing the diameter of two cells viewed with a ×40 objective lens calibrating a stage micrometer viewed with a ×10…

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Question 2 · 1 mark · 1 Cell structure

Which statement about a light microscope is correct? A As the distance to see two points as separate points decreases, the resolution also decreases. B A bacterium 0.2 μm in…

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Question 3 · 1 mark · 1 Cell structure

The electron micrograph shows a cell at a magnification of ×108 000. What is the actual maximum length of the cell? A 0.509 μm B 0.676 μm C 1.48 μm D 6.76 μm

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Question 4 · 1 mark · 1 Cell structure

The table compares some biochemical molecules of P, Q, R and S. chlorophyll DNA RNA peptidoglycan --- --- --- --- --- P ✓ ✓ ✓ ✗ Q ✗ ✓ ✓ ✗ R ✗ ✓ ✓ ✓ S ✗ ✗ ✓ ✗ Key: ✓ = present; ✗ =…

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Question 5 · 1 mark · 1 Cell structure

Which cell structures contain enzymes and are enclosed by a double membrane? 1 nucleus 2 mitochondrion 3 chloroplast A 1, 2 and 3 B 1 and 2 only C 1 only D 2 and 3 only

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Question 6 · 1 mark · 1 Cell structure

What is the order of size of cell structures? largest → smallest --- --- A centrioles → ribosomes → lysosomes → nucleoli B centrioles → nucleoli → lysosomes → ribosomes C nucleoli…

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Question 7 · 1 mark · 2 Biological molecules

A sample of a solution of sucrose tested for reducing sugars remains blue, but when another sample of the same solution is tested for non-reducing sugars the solution turns red.…

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Question 8 · 1 mark · 2 Biological molecules

Which molecules contain at least two double bonds? A Venn diagram shows three overlapping circles labelled 'saturated triglyceride', 'collagen', and 'haemoglobin'. Regions A, B,…

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Question 9 · 1 mark · 2 Biological molecules

Amylose, amylopectin and glycogen are all polysaccharides. Enzyme X removes one maltose molecule at a time from the ends of a polysaccharide molecule by hydrolysis of…

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Question 10 · 1 mark · 2 Biological molecules

Which property of water enables sweating to be an efficient means of losing heat from the body? A The latent heat of vaporisation of water is high. B The specific heat capacity of…

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Question 11 · 1 mark · 2 Biological molecules

Which bonds are found in the levels of structure of a protein molecule? primary structure secondary structure quaternary structure --- --- --- --- A covalent bond hydrogen bond…

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Question 12 · 1 mark · 3 Enzymes

Prokaryotes and eukaryotes use extracellular enzymes. Extracellular digestive enzymes are found in the external environments of some prokaryotes. Some prokaryotes are digested by…

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Question 13 · 1 mark · 3 Enzymes

Amylase breaks down starch molecules. Which substance will have the same number of molecules for the duration of this reaction? A amylase B water C maltose D amylose

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Question 14 · 1 mark · 3 Enzymes

Which graphs could show the effect of pH on the rate of enzyme-catalysed reactions? Four graphs are shown (1, 2, 3, 4) each with pH on the x-axis (1 to 14) and rate of reaction on…

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Question 15 · 1 mark · 3 Enzymes

After a heart attack, the enzyme lactate dehydrogenase leaks into the blood plasma from damaged heart muscle. What is required to measure the activity of lactate dehydrogenase…

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Question 16 · 1 mark · 3 Enzymes

Which graph correctly shows Km and Vmax? Four graphs (A, B, C, D) are shown. Each has 'rate of reaction' on the y-axis with Vmax and Km labelled, and a concentration variable on…

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Question 17 · 1 mark · 4 Cell membranes and transport

Cell membranes become less fluid as the temperature decreases. Bacteria and yeast cannot regulate their cell temperatures. How do bacteria and yeast maintain the fluidity of their…

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Question 18 · 1 mark · 4 Cell membranes and transport

Samples X, Y and Z are epidermal tissues cut from an onion. Each epidermal tissue was immersed in one of three different concentrations of a salt solution for 30 minutes. A…

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Question 19 · 1 mark · 4 Cell membranes and transport

The graph shows the results of an osmosis investigation using potato tissue. The y-axis shows percentage change in mass (from −20 to +20) and the x-axis shows concentration of…

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Question 20 · 1 mark · 4 Cell membranes and transport

Some agar was coloured pink using a pH indicator. The pink agar was then used to make three agar cubes with different dimensions: 2 cm × 2 cm × 2 cm, 4 cm × 4 cm × 4 cm, and 5 cm…

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Question 21 · 1 mark · 5 The mitotic cell cycle

The diagram shows the arrangement of chromosomes with attached spindle fibres during a stage of mitosis. The diagram shows chromosomes aligned at the equator of a cell with…

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Question 22 · 1 mark · 5 The mitotic cell cycle

During which phase of the cell cycle does DNA replication take place? A G₁ B G₂ C M D S

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Question 23 · 1 mark · 5 The mitotic cell cycle

Human body cells in interphase have 46 chromosomes. What is correct about the number of telomeres present in prophase of a human body cell? A 46 as there is one telomere at the…

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Question 24 · 1 mark · 6 Nucleic acids and protein synthesis

Sometimes hydrogen bonds form temporarily between the bases of two strands of RNA. How many hydrogen bonds form when guanine and uracil each bind to their complementary base?…

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Question 25 · 1 mark · 6 Nucleic acids and protein synthesis

The table shows all the possible DNA triplet codes for some amino acids. amino acid DNA triplet code --- --- cysteine ACA cysteine ACG tryptophan ACC isoleucine TAA isoleucine TAG…

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Question 26 · 1 mark · 7 Transport in plants

Which row is correct for transpiration? definition of transpiration comparison of water potential --- --- --- A evaporation of water from leaf surfaces higher inside air spaces in…

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Question 27 · 1 mark · 7 Transport in plants

Which features of companion cells and xylem vessel elements make them suitable for their function? companion cells xylem vessel elements --- --- --- A transport contents of cell…

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Question 28 · 1 mark · 7 Transport in plants

The diagram shows some root cells with arrows indicating the pathway of water moving from the soil. The water moves through the cell walls only (apoplast pathway), bypassing the…

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Question 29 · 1 mark · 7 Transport in plants

The graph shows the diameter of a tree trunk at different times. The x-axis shows time of day (00:00 to 00:00 spanning one full day). The y-axis shows diameter of tree trunk in…

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Question 30 · 1 mark · 7 Transport in plants

The plan diagrams of transverse sections of two plant organs are shown. These are not drawn to scale. The first organ (leaf-like shape) has Q labelling the upper…

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Question 31 · 1 mark · 8 Transport in mammals

The diagrams show three types of blood cells, which are all drawn to the same scale. Cell X is large with a kidney/U-shaped nucleus, cell Y is medium with a large round nucleus,…

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Question 32 · 1 mark · 8 Transport in mammals

The graph shows oxygen dissociation curves for three animals. The shape of each curve is influenced by respiration rate and the affinity of haemoglobin for oxygen. Animal 1…

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Question 33 · 1 mark · 8 Transport in mammals

Which statements about a mammalian heart are correct? 1 The right atrium and right ventricle contain deoxygenated blood going to the lungs. 2 The left atrium and left ventricle…

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Question 34 · 1 mark · 8 Transport in mammals

How is most carbon dioxide transported in the blood? A as carbaminohaemoglobin B as carbonic acid C as hydrogencarbonate ions D in solution in cytoplasm

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Question 35 · 1 mark · 9 Gas exchange

Which row shows the tissues that are present in the wall of the trachea and the wall of the bronchus? cartilage ciliated epithelium smooth muscle --- --- --- --- A ✓ ✓ ✓ B ✓ ✓ ✗ C…

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Question 36 · 1 mark · 9 Gas exchange

Which factors maintain the diffusion gradient for carbon dioxide at the surface of the alveoli? 1 blood flow around the alveoli 2 breathing movement exchanging air in the lungs 3…

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Question 37 · 1 mark · 10 Infectious disease

A student made notes about two diseases: hand, foot and mouth disease (HFMD) and Lyme disease. HFMD is caused by a viral pathogen and is transmitted in a variety of ways…

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Question 38 · 1 mark · 10 Infectious disease

Which statements explain why antibiotics may not treat cholera successfully? 1 The infectious organism has developed resistance to the antibiotic. 2 Antibiotics cannot harm…

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Question 39 · 1 mark · 11 Immunity

The statements describe the phagocytosis of a bacterium by a macrophage. 1 Digestive enzymes break down the bacterium. 2 Lysosome fuses with the vacuole. 3 Macrophage displays the…

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Question 40 · 1 mark · 11 Immunity

Two people, G and H, were each given an injection to protect them against a particular pathogen. One person was injected with antibodies. The other person was injected with a…

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