AS & A Level Biology (9700) — May–June 2025, Paper 14

40 questions · every question answerable online with instant point-by-point AI marking against the official mark scheme.

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Question 1 · 1 mark · 1 Cell structure

Plant cells were observed at ×400 magnification. Which structures could be clearly visible in these cells? A cell surface membranes B centrioles C nucleoli D ribosomes

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Question 2 · 1 mark · 1 Cell structure

The diagram shows a stage micrometer used to calibrate an eyepiece graticule. Y is the number of eyepiece graticule divisions and Z is the number of stage micrometer divisions.…

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Question 3 · 1 mark · 1 Cell structure

The diagram shows two stage micrometer scale readings used to measure pollen tube growth. The pollen tube grows 5 μm in one hour. What is the growth rate? A 5 μm h⁻¹ B 10 μm h⁻¹ C…

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Question 4 · 1 mark · 1 Cell structure

Which structures are found in both chloroplasts and mitochondria? 1 DNA 2 70S ribosomes 3 mRNA A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

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Question 5 · 1 mark · 1 Cell structure

Which structure in a muscle cell would increase in number in a person who trains regularly as a runner? A cell surface membrane B mitochondrion C nucleus D ribosome

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Question 6 · 1 mark · 1 Cell structure

Which functions are carried out by the Golgi body? 1 production of lysosomes 2 modification of polypeptides 3 synthesis of DNA A 1 only B 1 and 2 only C 2 and 3 only D 1, 2 and 3

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Question 7 · 1 mark · 1 Cell structure

A unicellular organism is found to have a nucleus, mitochondria and a cell wall. Which statement is correct? A The organism is not prokaryotic because it has a nucleus. B The…

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Question 8 · 1 mark · 2 Biological molecules

Boiled diastase is added to a starch solution. What would be observed when iodine solution and Benedict's reagent are added? A iodine = blue-black; Benedict's = blue B iodine =…

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Question 9 · 1 mark · 2 Biological molecules

Which substance is required for sucrose hydrolysis? A amylase B maltase C hydrochloric acid D sodium hydroxide

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Question 10 · 1 mark · 2 Biological molecules

A Venn diagram has three overlapping circles: unsaturated fatty acid, collagen, haemoglobin. A molecule with at least two carbon-carbon double bonds would be in which region? A…

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Question 11 · 1 mark · 2 Biological molecules

Which statement about globular proteins is correct? A They are insoluble in water. B They all have quaternary structure. C They can change shape using energy from ATP. D They all…

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Question 12 · 1 mark · 2 Biological molecules

Which diagram correctly shows a hydrogen bond between two water molecules? A [diagram showing H-bond between H of one molecule and O of another] B [diagram showing H-bond between…

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Question 13 · 1 mark · 2 Biological molecules

A detergent disrupts hydrogen bonds in water. What effect would this have on the specific heat capacity and latent heat of vaporisation of water? A both decrease B specific heat…

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Question 14 · 1 mark · 3 Enzymes

The graph shows the effect of temperature on the activity of compound X. Which row correctly describes compound X? A [enzyme-like, activity decreases above optimum] B [non-enzyme…

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Question 15 · 1 mark · 3 Enzymes

Enzyme activity was measured at different temperatures. Which statement is correct? A The enzyme has its optimum temperature below 30°C. B The enzyme has its optimum temperature…

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Question 16 · 1 mark · 3 Enzymes

Inhibitor X is a competitive inhibitor. The graph shows Michaelis-Menten curves with and without the inhibitor. What is the Km value in the presence of inhibitor X? A 0.12 mol…

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Question 17 · 1 mark · 4 Cell membranes and transport

Which substance can pass through the cell surface membrane without a protein? 1 glucose 2 CO₂ 3 Na⁺ ions A 1 only B 1 and 3 only C 1 and 2 only D 2 only

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Question 18 · 1 mark · 4 Cell membranes and transport

Which statement about facilitated diffusion is correct? 1 The rate is limited by the number of transport proteins. 2 It requires energy from ATP. 3 It can move molecules against a…

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Question 19 · 1 mark · 4 Cell membranes and transport

An enzyme is synthesised and secreted from a cell. What is the correct order of organelles involved? 1 cell surface membrane 2 Golgi body 3 rough endoplasmic reticulum 4 secretory…

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Question 20 · 1 mark · 4 Cell membranes and transport

The diagram shows apparatus used to investigate the movement of glucose molecules through a selectively permeable membrane (Visking tubing) at different initial concentrations (1%…

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Question 21 · 1 mark · 5 The mitotic cell cycle

The epithelium that lines the stomach is damaged by acid and is renewed every two days. Why is mitosis required to repair the damage? A Mitosis repairs damaged cells. B Mitosis…

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Question 22 · 1 mark · 5 The mitotic cell cycle

What is the role of telomeres? A allowing the chromatids to reach the poles during mitosis B holding sister chromatids together C making sure that the sister chromatids are of…

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Question 23 · 1 mark · 5 The mitotic cell cycle

Which features of mitosis help to maintain the genetic composition of the cell? 1 the longitudinal division of the centromeres 2 the DNA of the parent cells replicates before…

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Question 24 · 1 mark · 6 Nucleic acids and protein synthesis

DNA forms a leading strand and a lagging strand during semi-conservative replication. Which row correctly matches the strands to their properties? the strand that is replicated by…

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Question 25 · 1 mark · 6 Nucleic acids and protein synthesis

How many genes could code for one collagen molecule? A 1 gene only B 2 genes only C 3 genes only D 1 gene or 2 genes or 3 genes

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Question 26 · 1 mark · 6 Nucleic acids and protein synthesis

The diagram shows some events during the formation of an mRNA molecule at transcription. P = the gene; Q = the ends of the gene; S = sequences spliced out; R = the mature mRNA…

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Question 27 · 1 mark · 7 Transport in plants

Two plants, K and L, are parasites of other plants. Plant K grows into xylem tissue and plant L grows into phloem tissue. Which substances could plants K and L take from the…

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Question 28 · 1 mark · 7 Transport in plants

Which statements are correct for the apoplast pathway? 1 Water enters the cell wall. 2 Water moves by osmosis. 3 Water moves from cell wall to cell wall. 4 Water moves through…

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Question 29 · 1 mark · 7 Transport in plants

Mass flow is the bulk movement of materials from one place to another. Which vessels carry fluids by mass flow? 1 artery 2 phloem sieve tube element 3 vein 4 xylem vessel element…

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Question 30 · 1 mark · 7 Transport in plants

Which features are present in companion cells and also in phloem sieve tube elements? ribosomes plasmodesmata --- --- --- A present not present B present present C not present…

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Question 31 · 1 mark · 8 Transport in mammals

Which two terms describe the mammalian circulatory system? A open and double circulation B closed and double circulation C closed and single circulation D open and single…

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Question 32 · 1 mark · 8 Transport in mammals

One function of an arteriole is to increase or decrease the flow of blood to tissues. Which of these must be present in an arteriole wall to allow this function? 1 collagen 2…

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Question 33 · 1 mark · 8 Transport in mammals

The table shows blood pressures in different parts of the circulatory system: aorta = 16.0 kPa; arteriole = 11.3 kPa; arterial end of capillary = 4.7 kPa; venous end of capillary…

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Question 34 · 1 mark · 9 Gas exchange

Which reaction is catalysed by carbonic anhydrase? A the dissociation of carbonic acid B the formation of carbaminohaemoglobin C the formation of hydrogencarbonate ions and…

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Question 35 · 1 mark · 9 Gas exchange

What directly causes the percentage oxygen saturation of haemoglobin to decrease in actively respiring muscles? A hydrogencarbonate ions B carbon dioxide C carbonic acid D…

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Question 36 · 1 mark · 9 Gas exchange

Which row shows the tissues that are present in the wall of the trachea and also the wall of the bronchus? ciliated epithelium squamous epithelium smooth muscle --- --- --- --- A…

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Question 37 · 1 mark · 10 Infectious disease

Outbreaks of cholera commonly occur in camps set up after a natural disaster. Control measures to limit spread: 1 treating all drinking water supplies with a high concentration of…

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Question 38 · 1 mark · 10 Infectious disease

Tetracycline inhibits 70S ribosomes. Rifamycin inhibits prokaryotic RNA polymerase. Which antibiotics can be used to treat an infection caused by the influenza virus? penicillin…

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Question 39 · 1 mark · 11 Immunity

Which cells involved in the primary immune response are phagocytes? A monocytes B plasma cells C T-helper cells D T-killer cells

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Question 40 · 1 mark · 11 Immunity

Some ways in which different types of monoclonal antibodies can work: 1 binding to proteins on cell surfaces and triggering the immune system 2 blocking molecules on cell surfaces…

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